Race Relations in I Know Why the Caged Bird Sings by Maya Angelou Essay

Race Relations in I Know Why the Caged Bird Sings by Maya Angelou The reasons listed by the censors for banning I Know Why the Cage Bird Sings do not explain the widespread controversy around the novel. There is reason to believe that the question of the novel is in its poignant portrayal of race relations. This explains why the novel has been most controversial in the South, where racial tension is historically worst, and where the novel is partially set. Therefore, understanding the blatant and subtle effects of racism on the young Marguerite help explain the censorship controversy, and the person she became. One of the earliest examples of race relations in the book symbolizes the strict dichotomy of opportunity for black and white children. On the second page, Marguerite explains how she wished that she would wake up in a white world, with blond hair, blue eyes, and she would shudder from the nightmare of being black. Thus, from the beginning of the book, race relations were one of the major themes. Maya Angelou also shows the effect of oppression on the black people, and that impact on her as a child. One early example occurred when the po' white trash children confronted Mama in front of the store. They were represented as clownish, dirty, and rather silly. On the other hand, Mama simply stood like a rock and sang the Gospel. Her beauty of soul versus their disgusting antics creates a powerful scene about the nature of the oppressed and the oppressor. Marguerite, meanwhile, lies crouched behind the screen in agony at the inability of her class to command respect simply because of their color. Then, as the scene progresses, she understands that in spite of the disparity of power between the po'white trash and M... ...ice. It is interesting to note the poetical nature of I Know Why the Caged Bird Sings. Her perspective of a young girl is flawless. One truly sees the events through the eyes of a young girl. For example, the molestation scenes are depicted simply and innocently, which bothers one's consciousness. Another aspect of the book is the way in which the chapters are laid out. At the beginning of each chapter, Maya introduces a topic, discusses it, and then provides resolution. Each chapter is a short story by itself, but they also relate together. The chapters build on each other, and the end provides resolution to the common threads of the book. The end, however, also is a new beginning for Marguerite. It is the perfect ending to a profound and moving novel. Works Cited: Angelou, Maya. I Know Why the Caged Bird Sings. New York: Random House, 1969. Â  

Effects of Migration Into Cities

The effects of moving into a new city or town Today everybody is going in the glamorous world of cities where one can progress and make the best use of his or her life. Parents†¦.. Listen to their talks with their children. They say that my son or daughter will settle or go to abroad one day. And with this they start imagining and forcing their child to get highest degree and run away from where they are. Each parent today has this mentality that there is heaven like something in abroad and much else. There are many such examples to discuss. But for now one is enough I think.In terms of living a life in a new city there are many possibilities of advantages as well as disadvantages as can be seen in education, employment and settlement point of view. These three things can be much better improved in a city where there is much scope to put a step forward towards his or her aim. In a city each one who is settled there has only one intention to earn a gallon of paper notes and coins and live a luxurious and easy way of life. This luxurious life is expected by all people. But there is something called feeling of attachment.There is an attachment towards his or her native place. One cannot forget his or her childhood days or their neighbours and the love between them. Emotions are not allowed in a city or the town. Today since all the places are becoming industrialized there is no space for emotions but only profit. And because of this all the people are running after them. There is a great effect on especially the country. Since all the citizens are settled in abroad and the income they earn is of no use to the country, the country’s economic status lowers as compared to other countries.The newer generation is no longer staying in their native country specially India. India is full of corruption, they say. But it is not so. If the abroad based thinkers try, the situation can be changed. The major disadvantage of moving to a new city is the loss of attach ment to the native place and their neighbours. From birth we are attached to our villages or town, its nature, its culture and its people. But as we move to a new area, far from ours, we cannot accept its culture and people very easily. We cannot adjust there due to our unforgettable habits with our ulture. As we move to a new city, we have to accept the people, the culture, and their behaviors and accordingly we have to adjust. But it is not so easy. Since the people are keenly interested in earning money and luxurious life they are ready to do anything for this. According to me, I can’t accept the concept of moving to a new place permanently. Because it affects our native place or country. Earlier during the British rule all the intelligent brains of India were sent out to England or the other countries out of India.So that the foreign countries would progress better in terms of technology. But with this, India had to suffer in its progression. Today after British rule is n o longer still their mentality always exists in each Indian that to go to abroad but not to stay here in India. The aim of writing this article is to aware the people of not to go to abroad but it is better and much better to stay in your own country and sacrifice your talents to your people and their future. – Prachiti Ganpule SYBA, St. Xavier’s college, Mapusa Goa

AO2/A03 How Steve Brankin manages people, finances and resources with in Asidua Essay

AO2/A03 How Steve Brankin manages people, finances and resources with in Asidua In Asidua the management of people, finances and resources under Steve are central to business success. He undertakes this huge managerial task in a number of ways. Interviewing Steve gave me a great insight into these methods. Managing People Recruitment, Retention and Training of Employees. Employees in Asidua have to go through a short listing process and a job interview. Jobs are advertised on their website, Asidua.com kike the example in appendix 1 showing the job vacancy for an â€Å"Experience NET Designer†. Steve will also make sure that this job is advertised properly so people capable of doing the job can apply. Another method of recruitment overseen by Steve is the advertisements of job vacancies at career fairs for example the UUJ Engineering and IT Careers Fair which is held at Jordanstown University. (See appendix 2) at this event Asidua meet prospective graduates and placements to discuss future opportunities with Asidua. This is an excellent way of recruitment as Steve can see the level of employees that have just come out of higher education. This fair at Jordanstown is the perfect place for this as it would appeal to a number of high performing graduates. Advertising in relevant places for example, Universities and schools. On the Asidua website they offer graduates at Queen’s a scholarship worth up to  £25,000, which includes at least eight weeks paid summer work between each academic year, a guaranteed paid industrial placement in year three and after completion of industrial placement, the opportunity for paid part-time work during the academic year. (See appendix 3) This creates a positive corporate image for Asidua as it show they invest in education and Asidua believe it is important. It also means Steve can employ people straight out of university as they will understand Asidua’s aims and the graduates will be trained through the scholarship. Allocation roles and responsibilities to all levels of employees After advertising these posts, Steve starts the application process. For every job within Asidua completes a Job Description, Person Specification and a Job Outline, all these are part of the application process. Applicants use these to see whether they are suitable for the job, and whether certain qualities are met by the applicants. (See appendix 4 application form) Looking at the application it shows that employees within Asidua have high level of expertise and experience due to the large amount of space left on the form for information. The application form specifically asks about the applications’ personal interests professional organisations they have been involved in and it also asks them to mention skills they have gained. For all these sections there is a large box to fill out, this shows us that Steve is looking for people that have high expertise and also that they have an interest or a passion for something. This large amount of information is an efficient way of short-listing people suitable for the job in question. The applicant Curriculum Vitae is also further help in the application process. The Job Description shows the Job Outline and Personal Specification for an Experienced NET Developer in Asidua. You can download these from the Asidua website and they provide lots of information for the applicant. Looking at the Job Description it clearly shows that a lot is expected of the person applying. Asidua offer lots of employment benefits, for example a 5% bonus per annum, pension scheme, annual leave, salary sacrifice scheme (childcare vouchers), flexible working, sick absence, death in service and health insurance. These working conditions are very high are going to make staff very motivated and this is why Steve has these in place, and this is why in March 2011 Asidua became the first employer to be shortlisted twice for the prestigious University of Ulster Placement Employers of the Year Award. (See appendix 6) Being awarded this shows Steve strong managerial skills. Form looking at the Job Description it is clear that Steve isn’t just interested in their qualifications he pays great attention to their personal attributes, requiring employees to be open-minded, flexible, have good communication skills and being able to work as part of a team. This shows that Steve wants his employee’s qualities to create a very effective and profitable work force. Motivating and developing staff Once all this criteria has been met, it is very important that Steve keeps the motivating of staff at high levels. This is mainly done by investing in employee’s development at Asidua, making sure that they are all keeping strong communication to maintain morale. Methods of training that Mr Brankin will use will encourage employees to update their regular skills in a regular basis which will lead them to enhancing their overall performance. There are several motivational theories that we can look at. One of them is Maslow’s Hierarchy of Needs and Elton Mayo’s Theory. (See appendix 7 and 8) Mr Brankin doesn’t plan to motivate his staff under these theories, Human Resources will manage these. However many of the policies in these two theories may been shown, as long as they are relevant to Asidua when situations arise. By looking at these theories we can see how Steve as put them into practice in Asidua in many different ways. Strong communication within Asidua are how informal events such as their breakfast club, sports events and Christmas dinners. Employees will feel a sense of community when they are a part of these things. This shows that Mr Brankin and the Human Resources Department are using the correct methods similar to Elton Mayo’s theory, which clearly focuses on working conditions that are not affecting employee’s motivation. It is the employee’s interaction that gives them a sense of recognition. Setting clear objectives and receiving feedback A manager such as Steve Brankin uses close supervision, carefully dictating how his employees complete their jobs. The understanding that employees dislike work and responsibility and the only reason they are there is for the financial reward. Giving employees praise is a positive and effective  way to get employees working. Asidua do this by appraisal/performance management schemes. This enables objectives and training needs to be agreed at the start of the performance review cycle and reviewed as appropriate in the year. Steve has said in our questionnaire that feedback is an ongoing basis, and it is formally documented at the mid way point and at the end of the cycle. Another way Asidua set objectives is by setting task specifically suited to the employees skills, knowledge and experience, by doing this employees within Asidua will be given task that they are interested in and it means they will be completed to the best of the persons abilities. Maslow’s Hierarchy of Needs has been ignored in Asidua as Steve Brankin uses it to look at the management in Asidua. He uses this theory as it has the idea that needs are essential for employee’s motivation. Physical of basis needs are met within Asidua with, salary, shelter and warmth. The second main need is security, which employees within Asidua will also feel as they will be working in great conditions and the employees have job stability. Mr Brankin shows employees that the business is under his management and proves that Asidua are investing in their employee’s long-term employment. Staff development Staff development is very important in Asidua; this is because of employee’s long-term employment, which creates a sense of community within the business. Employee’s relationship is excellent in Asidua as they are all well known and this can lead to high levels of motivation. The cost of recruitment is lowered because of this as there are a lot of stages that need to be taken into consideration for example short-listing. So the better developed Asidua’s staff are the less employment is needed to replace past employees. Resolving conflict and maintaining morale Sense of belonging is important in Asidua. Steve puts a large emphasis on  the management style, the â€Å"open door† policy. This means that employees can openly and freely talk about their problems or concerns they may have or that may arise between employees, but all these issues can be resolved through training that managers receive to deal with these problems. Asidua have a Grievance and Harassment policy, this is where employees can report issues more formally. Opportunities with Asidua for employees to make decisions meet Maslow’s Hierarchy about self-esteem. Steve’s democratic management style means that this is being strongly met. Also employee’s decisions are listened to and not just thrown away. An example of this are the weekly meetings held in Asidua with Asidua with Mr Brankin and his employees. They also have the opportunity to feedback online, with different online forums. Employee’s opinions are valued, and they are a major part of the decision making process. Looking at how employees within Asidua are given freedom to carry out their own ideas, this shows that there is an extensive amount of training provided for the employees wanting to carry out their own projects or to press into the area that interest them the most. This management allows employees to keep their morale high as they have the freedom at work to do the things that interest them. Managing finance Steve’s management of finance is very important, just like any other management in Asidua it is clearly a very important factor of the whole business. Money that the business has and earns has to be distributed correctly thought out all the departments within Asidua. Without the correct finance management Asidua go into bankruptcy which is something Steve doesn’t want to happen to his company. When Steve is managing finance, decision making is crucial, the financial systems used within Asidua helping him make these important decisions. Forecasting Forecasting gives Asidua a way to manage its finances in a way that they can see future revenues and expenditures. It looks at three main sections: Receipts which Asidua expect to receive from sales. The payments of Asidua such as monthly costs including the rent and rates of their main business sites The net in/out amount of cash being overall difference between receipts and payments given an indication of how much money id left over by the end of the month. All this is vital to Steve so he can make sure any expected cash surpluses are used effectively and can also predict any future problems. Mr Brankin can then work to fix the problem so that they don’t get forced into liquidation. This method of forecasting means that business are able to evaluate any liabilities which need to be met and use the budgets from the facilitate the budget seen from this to facilitate Asidua in setting targets and then been able to measure performance against this by looking at variances which may arise in forecasting Steve can make important strategic decisions if from looking at Asidua’s cash flow forecast it can be seen that their assets will not be able to meet liabilities this will cause Steve to make immediate action showing this method is extremely useful for him in the financial decision making process. Cash flow management Cash flow statements will similarly be used this Mr Brankin to assist in this decision making process. This cash flow statement is the actual record of receipts and payments in the business as opposed to those which are forecasted the shows the actual journeys the cash has gone through in Asidua overtime and will be used by Steve to evaluate and compare these actual receipts and payments from the business with those forecasted in the cash flow forecast. It similarly uses a form of budget in Asidua as this record of cash flow in Asidua will allow Mr Brankin to plan any future cash flow requirements and  allow him to make sure cash surpluses are used affectively. Like forecasting if cash flow is not managed effectively the business could go into administration. Steve will also use profit and loss accounts to manage finance. Profit and loss accounts are basically a summary of the business is transaction over a given period of time normally 12 months deduction the total expenditure from the total income. It is different from cash flow statement and forecasting in the way that is produced primarily for business purposes to show Asidua’s stakeholders and potential partners in Asidua. This information is obtained by HM Revenue and Customs to solve out taxation. This account also give Steve a way to look at the business’ performance monitoring whether Asidua’s actual performance is in correspondence with budgets. Financial decision making this is similar to profit and loss accounts as it shows the businesses liabilities and assets in balance sheets. These are different as they show the value of Asidua on a particular day, the idea of this is to make sure that every day thing is balanced with Asidua. This means it must be updated constantly. However it is a brilliant method of finance management adopted by Steve Brankin. Making day-to-day financial decisions is very important. Profits and break even analysis. Giving advice on capital investment There are many different financial methods which Steve has not adopted. One of these is break even analysis. Break even is when Asidua’s total revenue is equal to their total cost, by known the point these two meet is very important for Steve as he can aim to meet this break even point. This is the number of items which have to be sold to make a profit. It can also be used to avoid making a loss. This is because any amount of product sold under this point would mean that there is a loss made and cost would not be covered. There is a margin of safety within break even analysis; this is very useful as it shows the difference between the number of goods Asidua would have sold between the break even point and the selected level of activity. This  is very practical for Asidua as it monitors how they are making a profit. When managing finance the decision of capital investment is also very important. Mr Brankin must invest time into looking at this as capital is very important factor of production. When Steve decides whether or not to invest capital into Asidua a number of factors must be thought of. For example when Steve puts forward the business plans to continue investment in Northern Ireland’s young talent, which they got three new scholars to join Asidua through their scholarship program. Those participating in the program had to go through serious high levels of application. This has its benefits as coming from this investment into the support of new IT and engineering student; this will lead Asidua to high profit and success great than the initial cost of the program. Liaising with outside agencies e.g. Inland Revenue Steve makes sure that Asidua liaises with several companies within Asidua; this is mainly done for financial reasons. Asidua have links with Inland Revenue, they bring together the direct taxes and other duties of the business. It is important that areas for example finance is handled by specialists. The investment with Inland Revenue is very effective as they provide Steve with professional financial support. Managing other resources. Stave is in charge of the information systems within in Asidua. These are crucial for a business such as Asidua to run smoothly. Information systems It is evident that Asidua and Steve use information systems and are heavily involved in them. As a company that specialise in computers they used computer aided design to manage most of their information and capture diagrams. Desktop publishing is also used in Asidua to manage information. This is used in lots of businesses and is the software to create publication documents on a computer. This allows large amounts of books to be published  and this method enhances Steve’s managerial skills for information. Linux operating systems is also used within Asidua. This operating system can be installed on a wide variety of computer devices including mobile phones. This shows that this operating system is very effective and Steve can use it in many ways. Maintenance and renewal assets Asidua’s website is one of their assets as it allows their stakeholders and those within Asidua to find out about the business, the employees, projects under consideration and events. This is an excellent way to promote the business as this is based on the high quality of the website and it is important for the business’s image. Apart from Asidua’s employees having the benefit of being able to be updated via Asidua’s public web page, the business also have an intranet system in place. This system is Asidua’s private network created for employees which enables them to find items in their website easier. This has its benefits as it motivates employees and improves morale; this asset provided by Asidua must be renewed and updated regularly under Steve’s management for success.

Top Gear Reviwe

As Jeremy Clarkson explained on Twitter, â€Å"fat people singing† has been in Top Gear’s way for the past year. So it’s ironic that the programs return opened with the overweight presenter screeching â€Å"we’re back! † with all the keenness of a desperate X Factor finalist. This is where comparison between padded-out talent contestants and Top Gear end; however the free-wheeling motoring show was very nearly a full hour of fun, witty entertainment. Top gear was officially complained about by the Indian High Commission on its recent trip to India.Jokes about terrorism, the Nazi regime and incest in tonight’s episode suggest Top Gear’s tone remained unchanged by critics and complainers. The banter between Top Gear trio Richard Hammond, May and Clarkson just great, May was old; Hammond’s choice of clothes was laughable, Clarkson moaned about motorway signs in his usual comic way. The Homeland’s star Damian Lewis was the â €˜Star in a Reasonably Priced Car’, who dropped names and great story’s (playing at Old Trafford, surviving a motorcycle accident) with genuine humour, which was much needed, because a few jokes seem a bit manufactured. Top Gear thrived outside of its dull studio.We saw Hammond yell theatrically inside a â€Å"savage† supercar: the Pagani Huayra is worth ? 800,000 and now takes its place at the top of the Power Lap Board – which set the bar high for the rest of the series. Eagle-eyed fans will have seen September’s leaked footage of May co-driving a Bentley continental GT Speed on a WRC rally stage. Tonight’s full feature was one of dramatic skies, dark forests and a tough lesson for him in rally driving (rally pace notes: directions given in hasty anticipation to the driver). â€Å"Either get it right or shut up†, growled May’s quietly, terrifyingly soiled professional driver.Pleasingly, the Bentley’s four-wheel driv e dealt well with a proper thrashing. Enthusiastically, Clarkson showed us his self created ‘P45’. The vehicle (it can hardly be called a car) aims to be smaller than the Peel P50 – a 1960s micro mobile invented for city driving. Clarkson, just shy of two metres tall, was transformed into a grumpy toy robot in the weird contraption, driving along country lanes, bombing down dual carriageways and sneaking into shopping centres with pleasant hilarity. Add in some comical humour, serious cool cars and it’s like top gear never went away.

Development of Egyptian Pyramids from Sumerian Ziggurats Essay

The Egyptian pyramid structures represent a great range of civilization techniques that have been used in various other parts such as in Rome and Greece. This is despite the fact that the eventual architectural construction of pyramids in Egypt took place in slow transitional steps during the 3rd transition to a unique Egyptian character and permanency in the 4th dynasty although the â€Å"idea was brought to Egypt by the Mesopotamians† (Isler 90). The original idea came from the structures of the Ziggurats that are of various sizes and whose bases range from 20 meters on the side and 90 meters on a side. They are usually very high temples built on a platform of adobe brick with a series of platforms that create a stepped pyramid. They are therefore of major importance as they are mainly used for city planning and are dedicated to the god or goddess of the city’s patron. This is a major form of civilization which was introduced in Mesopotamia through the epic journey of Gilgamesh in 2700 B. C who later built the city of Uruk (Hooker par. 3-6). His account which was inscribed in some of the oldest and earliest tablets existed before those provided on the pyramid texts. Since the structure of ziggurats does not have a specific definition, the staged towers which possess consciously constructed stages are generally referred to as ziggurats. They are mainly found in the area of Mesopotamia. The siak ziggurat dates back to 5500 B. C although it is not yet clear what was anciently used to classify structures as ziggurats. Most temples on accumulated ruins were the original staged towers even though the stages which were made of accumulated ruins were not constructed for the tower. Hence the structures are only referred to as ziggurats when the stages are constructed and modeled after the piled up ruins. Some of their functions arise since they were built as a representation of mountain to offer protection for the temple against floods, and religiously they appear in the form of unity since they form a place where the gods dwell. There are a number of evidences that the Egyptian pyramids borrowed their building techniques from the Sumerian ziggurats. For instance, the ziggurats â€Å"and typically the elamite ziggurat exhibit a striking resemblance â€Å"(Isler, 32) and are similar in shape to the early Egyptian pyramids most especially the step pyramid of Djoser at Saqqara except that they do not â€Å"possess a sanctuary at its apex and had a system of internal tunnels and chambers† (World Mysteries par. 3) . In addition, they date some years later than the earliest ziggurats as the great pyramid dates back to around 2500 B. C. The architectural forms of the pyramid began as a simple mastaba which was built in several stages. This step pyramid is a product of the â€Å"Egyptian third dynasty which was simultaneous with the early dynastic period in Mesopotamia† (Walton par. 1-5). However, they are built by use of mud bricks, a technique developed in Palastine in the Neolithic period and not in Mesopotamia. The civilization in Egypt which is accompanied by the technique of building pyramid tombs is a sign of concern about death and the search for immortality. This is because they were worried about the nature of death and the hope for survival after death, a factor realised by Gilgamesh when he discovers â€Å"the fateful truth that death awaits every person† (Smart 201-203). Works Cited Hooker, R. Gilgamesh. 6 June 1999. 24 August 2010 . Isler, M. â€Å"Sticks, stones, and shadows: building the Egyptian pyramids. † USA, Oklahoma: University of Oklahoma Press, 2001. 32-90. John H. Walton. Is there archaeological evidence of the Tower of Babel? 2001. 24 August 2010 . par. 1-5 Smart, N. â€Å"The World’s Religions, 2nd Ed. † USA: Cambridge: Cambridge University Press, 1998. 201-203. World Mysteries. The Age of the Great Pyramid. 2009. 24 August 2010 .

Biochemical Action of Bacteria

OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a sample of different chemical. The testing could be focused on a specific type of bacteria, medical bacteria or a broad range of environmental bacteria.Since bacteria are present in virtually any environment, it’s important to be clear why the testing is being performed. The more specific the testing is the better and the easier it is to interpret the results. Numbers and types of bacteria that should be a cause for concern depends upon several factors, including the type of bacteria present and t he type of samples. Escherichia coli  are one of the main species of bacteria living in the lower intestines of mammals. E. coli  can be found in the intestinal tract of warm-blooded animals. The presence of  E. coli  in foods is considered to be an indication of fecal contamination.Staphylococcus  organisms are commonly found in the environment. Several species of  Staphylococcus  are found on the skin, intestines, nasal passages, etc. of warm-blooded animals. Some species of  Staphylococcus, particularly  Staphylococcus aureus  can be pathogenic are capable of causing illness. Pseudomonas aeruginosa is widely distributed in soil, water and plants. It survives in hot tubs, whirlpools, contact lens solution, sinks and showers. It can cause a number of opportunistic infections including infections of the skin, external ear canal and of the eye.Nitrifying bacteria recycle organic nitrogenous materials from ammonium (the endpoint for the decomposition of proteins) t o nitrates. Their presence can indicate that the water may have been polluted by nitrogen-rich organics from sources such as compromised septic tanks, sewage systems, industrial and hazardous waste sites and is undergoing an aerobic form of degradation. The presence of denitrifying bacteria can indicate that the water has been polluted by nitrogen-rich organics from sources such as compromised septic tanks, sewage systems, industrial and hazardous waste sites. MATERIALS: 1. Nutrient broth cultures of Escherichia coli . Nutrient broth cultures of Serratia marcescens 3. Nutrient broth cultures of Salmonella typhimurium 4. Nutrient broth cultures of Bacillus subtilis 5. Nutrient broth cultures of Klebsiella spp. 6. Nutrient broth cultures of Streptococcus spp. 7. Nutrient broth cultures of Staphylococcus aurieus 8. Nutrient broth cultures of Proteus vulgaris 9. Nutrient broth cultures of Pseudomonas fluorescens 10. Parafilm tape 11. Inoculating loops 12. Gloves 13. Incubator 14. Nutrie nt agar plate 15. Nutrient agar slants 16. Starch agar plates 17. Gelatine agar plates 18. 2 tubes Clark’s-Lub medium (MR-VP medium) 19. Tryptone broth 20. 3 Kigler’ slant 21. 5 tubes nitrate broth ( 0. 1% KNO3) 22. 5 urea broth 23. Tube containing 10ml of sterile saline 24. Glucose broths with Durham tubes and phenol red indicator 25. Lactose broths with Durham tubes and phenol red indicator 26. Sucrose broths with Durham tubes and phenol red indicator 27. Gram’s iodine 28. Kovac’s indol reagent 29. Mercuric chloride solution 30. KOH-creatine solution or 40% KOH 31. F&R reagent 32. Nessler’s reagent PROCEDURE: A. CARBOHYDRATE METABOLISM 1. Fermentation of sugars Materials: 1. Glucose broths with Durham tubes and phenol red indicator 2.Lactose broths with Durham tubes and phenol red indicator 3. Sucrose broths with Durham tubes and phenol red indicator 4. 18 hour nutrient broth cultures of E. coli and S. typhimurium Procedure: 1) The small bottles of different sugars were inoculated with a loopfuls of E. coli and Salmonella spp. 2) The tubes were labelled and incubate at 37oC for 24 hours 3) All observations were recorded for presence of acid or gas production. 2. Hydrolysis of starch Materials: 1. Starch agar plates 2. Broth agar cultures of B. subtilis and E. coli Procedure: 1) Starch plate was streaked with E. coli in for sections and repeated for B. ubtilis bacteria in other starch plate. 2) The plates were secured with parafilm, labelled and inoculated at 37oC for 24 hours. The following day 1) The plates were tested for starch hydrolysis by flooding the pates with Gram’s iodine. 2) The plates were examined and the colonies that showed clear uncoloured zones in contrast with the blue-black background of the starch-iodine complex were noted. 3) The extent of the zones of hydrolysis indicated either the reddish colour zones were seen. 4) All results and observations were recorded. B. PROTEIN AND AMINO ACID METABOLIM 1. Indole test Materials: 1. Broth cultures of B. ubtilis, E. coli, and S. typhimurium 2. 3 tubes of tryptone broth 3. Kovac’s indole test reagent Procedures: 1) The peptone water was inoculated with a loopfuls of the test organism. 2) The tube was labelled and incubated for 24 hours. The following day 1) The tubes were added with a few drops of Kovac’s indole reagent (dimethylaminobenzaldehyde) 2) The red or dark color indicates the presence of indole. 4. Hydrogen sulphide Materials: 1. Broth cultures of B. subtilis, E. coli, and S. typhimurium 2. 3 Kigler’s slant Procedures: 1) The Kigler’s slant was inoculated with a loopfuls of the test organism by the stab method. ) The tube was labelled and incubated for 24 hours. The following day 3) The Kigler’ slant was observed for production of H2S where the black precipitate along the line of growth in the Kigler’s slants indicated the H2S have been produced. 4) The observations were recorded. 3 . Gelatine hydrolysis test Materials: 1. Broth cultures of B. subtilis, E. coli, and S. typhimurium 2. Gelatine agar plates 3. Mercuric chloride solution Procedures: 3) The gelatine agar plates were inoculated with a loopfuls of the test organism with a single streak at the centre of the plates. ) The plates were secured with parafilm, labelled and incubated for 24 hours. The following day 5) The plates were flooded with mercuric chloride solution. 6) The medium become opaque in regions that still contain gelatine and clear regions where gelatine has been hydrolysed. C. VOGES-PROSKAUER TEST Materials: 1. Broth cultures of E. coli, and Klebsiella spp. 2. 2 tubes of Clark-Lub’s medium (MR-VP medium) 3. KOH-creatine solution Procedures: 1) The tubes of Clark-Lub’s medium (MR-VP medium) were inoculated with a loopfuls of the test organism. 2) The tubes were labelled and incubated for 24 hours.The following day 1) The tubes were tested with Voges-Proskauer test. 2) The 0. 5 ml of KOH-creatine solutuin was addd. 3) The tube was shaked vigorously for 30 seconds. 4) The red or pink color indicates the presence of acetoin. D. CATALASE TEST Materials: 1. Broth cultures of Streptococcus spp. and Staphylococcus aureus. 2. Nutrient agar slant Procedures: 1) The nutrient agar slant was inoculated with a loopfuls of the test organism. 2) The tube was labelled and incubated for 24 hours. The following day 1) The tubes were tested with catalase test by adding several drops of a 5% solution of hydrogen peroxide. ) The vigorous bubbling indicates the presence of oxygen. E. NITRATE REDUCTION TEST Materials: 1. Broth cultures of E. coli, Proteus vugaris, Serratia marcescens, Pseudomonas fluorescens. 2. 5 tubes containing nitrate broth (0. 1% KNO3) 3. Nitrate test reagent Procedures: 1) The nitrate broth was inoculated with a loopfuls of the test organism. 2) The tube was labelled and incubated for 24 hours. The following day 1) The tubes were tested with 1ml of Follet and Ratcliff’s (F&R reagent) 2) The orange or brown color indicates the presence of nitrate. 3) The absent of nitrate indicates that: a.There has been no nitrate reduction b. The reduction has proceeded beyond that nitrate stage. 4) The absent of orange or brown color were further tested with small amount of cadmium to the tube. If nitrate still present, it will be catalytically change to nitrate which will then reacts with the F&R reagent in the tube. 5) In the absent of a positive nitrate result, the bubbles f H2 gas was observed in the Durhams tube OR 6) The samples were tested with 1ml of Nessler’s reagent. The brown or orange color indicates the presence of ammonia. F. UREASE TEST Materials: 1. Broth cultures of E. coli, P. vugaris, S. arcescens, P. fluorescens. 2. 5 urea broth with indicator Procedures: 1) The urea broth was inoculated with a loopfuls of the test organism. 2) The tube was labelled and incubated for 24 hours. The following day 1) The urease-posit ive organism produced in intense red/purple coloration of the medium after incubation. 2) All observations were recorded. RESULTS AND OBSERVATION: Test| Observation(After 24 hours incubation)| Description| A. Carbohydrate Test 1. Fermentation of starchDurham tubes and phenol-red indicator. 2. Hydrolysis of starch| Glucose: Lactose: Sucrose: Starch agar plates:B. ubtilisE. coli| * Positive result for E. coli as tube turn yellow * Positive result for S. typhimium as tube turn yellow * Positive result for E. coli as tube turn yellow * No gas produced by S. typhimium because the tube turns red. * No gas produced by E. coli because the tube is slightly red. * Positive result for S. typhimium as tube turn yellow * Positive zone of clearing. * Negative zone of clearing. | B. Protein And Amino Acid Metabolism 1. Indole test 2. Hydrogen disulphide 3. Gelatine hydrolysis test| Tryptone broth:B. subtilisE. coli. S. typhimuriumKigler’s slant:B. subtilisE. oli. S. typhimuriumGelatine agar plates:B. subtilisE. coli. S. typhimurium| * Negative Indole tests no color change. * Bright fuschia at the interface is positive test for Indole. * Negative Indole tests no color change. * Black precipitate form shows positive sulphur reduction. * Negative reaction. * Positive reaction forming the black precipitate. * Positive hydrolysis of gelatine into amino acid to be used as nutrients/gelatinase. * Negative hydrolysis of gelatine. * Negative hydrolysis of gelatine| C. Voges- Proskaeur’s Test| MR-VP medium:E. coli. Klebsiella spp. | * Negative results of E. oli * Positive results Klebsiella spp. | D. Catalase Test| Nutrient agar slant:S. aureusStreptococcus spp. | S. aureus * Positive catalase reaction because present of bubblesStreptococcus spp. * Negative catalase reaction no bubbles present. | E. Nitrate Reduction Test| Nitrate broth:E. coliP. vulgarisS. marcescensP. fluorenscens| * No color change after denitrification of ammonia. * No color change after denitrificat ion of ammonia. * Turns red. Positive nitrate test shows nitrate reductase present. * Turns red but negative catalase test. | F. Urease Test| Urea broth:E. coliP. vulgarisS. marcescensP. luorenscens| * Negative urease test because the tube remain purple. * P. vulgaris show positive urease test from yellow to pinkish. * S. marcescens show negative urease test because the color remain purple. * P. fluorenscens show negative urease test because the color remain purple. | DISCUSSION: Biochemical tests of bacteria oobjectively to test the metabolism of carbohydrate and related products of different bacteria species, test specific breakdown of products through color changes and gas produced. Besides that, the ability of bacteria utilizes a specific substance and the metabolism of protein and amino acid by bacteria.A. CARBOHYDRATE TEST Carbohydrate is an organic compound that consists of only carbon, hydrogen and oxygen which is basically the major carbon source of most organisms. Specific carbohydrate can be fermented by organism that incorporated in a medium producing red or acid with gas. Pinkish red color shows positive results where acidic content formed in the tube because carbon dioxide realised if fermentation occur. Negative catabolism of carbohydrate shows by yellow to colourless of Durham’s tube as the solution remain alkaline in the absent of carbon dioxide gas.Gas production can be seen as bubbles in Durham’s tube. Central carbohydrate metabolism or the breakdown of sugars into smaller compounds accompanied by the production of ATP and reduction of coenzymes, follows one of several pathway. Carbohydrate utilization and fermentation will be assessed by growing cells without shaking (aeration) in defined media containing a single carbohydrate. Acid products of sugar fermentation will cause a noticeable color change in the pH indicator included in the medium.Sugar fermentation does not produce alkaline product, however non-fermentative hydroly sis of amino acids in the peptone, present in most fermentation media, may give an alkaline reaction, which will also cause a color change in the pH indicator. Gas production, H2 in particular, can be determined by placing a small, inverted Durham tube in the test medium. If gas is produced, it is trapped in the Durham tube and can be seen as a bubble. Hydrogen sulfide (H2S) is produced by bacterial anaerobic degradation of the two sulfur-containing amino acids, cysteine and methionine.Hydrogen sulfide is released as a by-product when carbon and nitrogen atoms in the amino acids are consumed as nutrients by the cells. Under anaerobic conditions the sulfhydryl (-SH) group on cysteine is reduced by cysteine desulfurase. Ferrous ammonium sulfate-indicator. H2S reacts with ferrous sulfate forming the black precipitate Sodium thiosulfate is reduced to sulphite/thiosulfate The Kligler's Iron test is used to detect liberation of H2S gas by bacteria growing on an excess of these sulfur-cont aining amino acids. The agar contains high levels of peptones or sources of cysteine and methionine and ferrous sulfate as an indicator.When H2S is produced, the ferrous ion reacts with it to give ferrous sulfide, an insoluble black precipitate. In starch hydrolysis test Iodine must be on the plate to visualize the zone of clearing surrounding the bacteria. This zone indicates starch was broken down to dextrins, maltose, and glucose. B. PROTEIN AND AMINO ACID METABOLIM Indole test measures the ability of bacteria to split indole from tryptophan molecule but in term of biochemistry, Indole test is one of the metabolic degradation products of the amino acid tryophan.Bacteria that possess the enzyme trytophanase are capable of hydrolysing and deaminating tryptophan with the production of Indole, pyruvic acid and ammonia. Positive reaction showed by E. coli, P. vulgaris and negative results observed in Klebsiella and Salmonella from observation in the Indole test. Development of fuchsia red color at the interface of the reagent and the broth within seconds after adding the reagent is indicative of the presence of Indole and is a positive test. Kovac’s reagent detects if tryptophan has been hydrolyzed to indol or tryptophanase.Gelatin is the protein derived from the animal protein collagen, has been used as a solidifying agent in food for a long time besides nutrient gelatine as an early type of solid growth medium. One problem is that many bacteria have the ability to hydrolyze or liquefy the gelatin. This gelatin liquefaction ability forms the basis for this test. C. VOGES-PROSKAUER TEST The production of acetoin by bacteria is perform through Voges Proskauer Test to determine the ability of the organisms to produce neutral end product acetyl methyl carbinol (acetoin) from glucose fermentation.Negative results gained from E. coli meanwhile positive reaction gives by. Changing of color to red pinkish color at the surface of the medium indicated positive res ults and yellow color at the surface of the medium show negative reaction. The KOH reagent should not be excessively added to the sample because excess KOH may mask weak VP positive reactions. The MR test will be positive for organisms that have complete pathways for mixed acid fermentation. The Voges-Proskauer (VP) test determines whether a specific neutral metabolic intermediate, acetoin, has been produced instead of acid from glucose.Acetoin is the last intermediate in the butanediol pathway, which is a common fermentation pathway in B. subtilis. The tests are complementary in the sense that often a bacterium will give a positive reaction for one test and a negative reaction for the other. The three possible patterns of results where the acetoin fermentation pathway, detected by the VP test, two molecules of pyruvate condense and two molecules of CO2 are released. The 4 carbon intermediate that is formed, acetoin, contains a carbonyl group. The acetoin acts as a terminal electron acceptor with the carbonyl group being reduced to a hydroxyl group.The reduced product, butanediol, is excreted by the bacteria and acetoin is oxidized to diacetyl by alkaline -naphthol, which forms a red complex with creatinine. D. CATALASE TEST Catalase is present in most cytochrome containing aerobic and facultative anaerobic bacteria except Streptococcus spp. Hydrogen peroxide forms as one of the oxidative end product of aerobic carbohydrate metabolism. If hydrogen peroxide allowed accumulating in the bacterial cells it becomes lethal to the bacteria. Catalases help in converting H2O2 to water and oxygen.In the catalase test performed, Streptococcus spp gives negative reaction as for S. aureus, the positive reaction occurred. One of the by-products of oxidation-reduction in the presence of O2 during aerobic respiration is hydrogen peroxide (H2O2). This compound is highly reactive and must be degraded in the cytoplasm of the cell producing it. It can be especially damaging to mo lecules of DNA. Most aerobes synthesize the enzyme catalase, which breaks down H2O2 into water and oxygen. The O2 gas is identified by the production of bubbles from a concentrated cell suspension.The test for catalase is simple and usually very reliable. It is a major method of distinguishing between Staphylococcus (catalase positive), Streptococcus (catalase negative), and Enterococcus (catalase negative), although some strains of Enterococcus faecalis may be positive. Catalase production is generally associated with aerobic organisms, since H2O2 is a toxic by-product of aerobic growth, but not always. E. NITRATE REDUCTION TEST Nitrate reduction test basically test the ability of organism to reduce the nitrate to nitrites of free nitrogen gas.In order to determine either the bacteria can reduce nitrate, the test organism is inoculated into nitrate reduction broth, undefined medium that contains large amounts of nitrate (KNO3). After incubation, reagent added simultaneously reacts with nitrite and turn to red color, indicating a positive nitrate reduction. If there is no color change at this step, nitrite is absent. If the nitrate is unreduced and till in its original form, this would be a negative nitrate reduction result. However it is possible that the nitrate was reduced to nitrite but has been further reduced to ammonia or nitrogen gas.This would be recorded as positive nitrate reduction result. Under anaerobic conditions, some bacteria are able to use nitrate (NO3-) as an external terminal electron acceptor. This kind of metabolism is analogous to the use of oxygen as a terminal electron acceptor by aerobic organisms and is called anaerobic respiration. Nitrate is an oxidized compound and there are several steps possible in its reduction. The initial step is the reduction of nitrate (NO3-) to nitrite (NO2-). Several possible products can be made from further reduction of nitrite. Possible reduced end products include the following N2, NH3 (ammonia), N2O (nitrous oxide).Bacteria vary in their ability to perform these reactions, a useful characteristic for identification. A medium that will support growth must be used and the cells must be grown anaerobically. Growth in the presence of oxygen will decrease or eliminate nitrate reduction. There are many possible end products of nitrate reduction such as nitrite, nitrogen gas (N2), nitrous oxides, ammonia, and hydroxylamine. The disappearance of nitrate or the appearance of the end products. The test relies on the production of nitrous acid from the nitrite. This, in turn, reacts with the iodide in the reagent to produce iodine.The iodine then reacts with the starch in the reagent to produce a blue color. Since some of the possible products of NO3- reduction are gaseous, a Durham tube is sometimes inverted in the culture tube to trap gases. This being the case, it is important to pre-test the medium to ensure no detectable nitrite is present at the beginning, and, in the case of a neg ative test, to reduce any nitrate to nitrite to determine whether the nitrite was also reduced. If nitrite is produced, it reacts with hemoglobin to give a bright red color, instead of the dark red color of hemoglobin.It is this reaction that is responsible for the color of meats, such as hot dogs, which are preserved with sodium nitrite. The blood agar test has the advantage of no color change occurring if the nitrite is further reduced. F. UREASE TEST Urease test mainly highlighted to determine the ability of the organism to split urea forming 2 molecules of ammonia by the action of the enzyme Urease with resulting alkalinity. Negative reaction shown by E. coli meanwhile Klebsiella spp. shows positive result. Extra precaution needed because both the urease test medium depend upon the demonstration of alkalinity that not specific for urease.Moreover the protein hydrolysis may result I alkalinity hence false positive may be seen in Pseudomonas. The false positivity can be eliminated by control test using the same medium without urea as recommendation. Urea is a nitrogenous waste product of animals. Some bacteria can cleaved it to produce carbon dioxide and ammonia. The ammonia is a nitrogen source for amino acid biosynthesis as well as for synthesis of other nitrogen-containing molecules in the cell. The urease test was devised to distinguish Proteus species from other enterics.The medium described here is buffered enough so that weak urease producers appear negative. The production of ammonia raises the pH of the medium. The indicator phenol red is present in the broth. Phenol red is orange-yellow at pH below than 6. 8, and turns bright pinkish-red at pH higher than 8. 1. Hence, a positive urea test is denoted by the change of medium color from yellow to pinkish red. CONCLUSION: Based on the laboratory, different bacteria species have different abilities to metabolize various substrates and end products formed were able to be observed and distinguished.

Commercial contracting Essay Example | Topics and Well Written Essays - 6000 words

Commercial contracting - Essay Example For advising Transco regarding these issues, the following discussion, relating to the terms used in the letters from Minerva and Hedge Plc have been taken up for discussion. Essay 1 Subject to Contract The formation of contracts is a process that is subject to continual change. This process has gradually acquired considerable intricacy and involves much higher stakes. Nevertheless, some features of this process have remained unchanged. For instance, the negotiators are interested at reaching an agreement and are keen to gain the maximum profit from it. These objectives are inherently contradictory; because, a party that is interested in effecting an agreement might be required to behave in a manner that diminishes its profits.1 It has been a long standing practice to employ the device of subject to contract in contractual negotiations. This expedient prevents the emergence of contractual liabilities, during contractual deliberations. Its use in correspondence that transpires before a contract is formed connotes the absence of contractual liability till such time as a contract is formed.2 The subsequent conduct of the parties to an agreement, primarily determines the outcome of the condition subject to contract. ... Furthermore, this term cannot be employed to indicate that no binding contract can be formed. In addition to the written words and negotiations the conduct of the parties also determines the existence of a binding contract.4 As a result, any instruction to commence work, prior to arriving at a final agreement, should be dealt with great care. In Regalian Properties plc v London Dockland Development Corpn [1995] it was held that an offer that had been accepted, subject to contract was not binding in nature. In this case, the plaintiff was a property developer who made its offer via a letter entitled subject to contract. 5 Due to difficulties envisaged in acquiring the land by the defendant and other reasons, the contract proved difficult to materialise. The plaintiff claimed heavy damages, which the court rejected, as the work had been undertaken on the basis of subject to contract, which implied the absence of a contract betwixt the plaintiff and the defendant. The plaintiff had incu rred considerable expenditure, in the anticipation of procuring the contract. The court held that the plaintiff’s actions had been at its own risk, as there was no binding contract between the plaintiff and the defendant.6 Thus, subject to contract provides a stratagem for circumventing liability in such claims. In Confetti Records v Warner Music UK Ltd [2003], it was ruled by the court that the inclusion of a track from the album did not constitute an infringement of copyright. The inclusion of the term subject to contract indicates that the party has no intention to confer a binding nature on deal memo. However, the act of sending the track and invoice by the claimants was to be considered a valid offer. If the defendant had